Volume 7, Issue 1 (3-2018)                   3dj 2018, 7(1): 43-50 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rastin V, Azizi B, Modabbernia S, Fayazi N, Rahimi M. Evaluation of Periodontal Disease Effect on the Expression of Vascular Endothelial Growth Factor and Nerve Growth Factor in Dental Pulp. 3dj 2018; 7 (1) :43-50
URL: http://3dj.gums.ac.ir/article-1-305-en.html
1- Assistant Professor, Department of Oral & Maxillofacial Pathology, Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2- Assistant Professor, Department of Periodontics, Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran. , bahareazizi64@gmail.com
3- Assistant Professor, Department of Oral & Maxillofacial Pathology, Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
4- Department of Oral & Maxillofacial Pathology, Faculty of Dentistry, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Abstract:   (2338 Views)
Introduction: Researchers have always been interested in the close and mutual relationship between the pulp and periodontal tissues. Although the effects of pulpal disease on periodontium have been well known, the clear relationship between periodontitis and pulp has not been documented . It seems that bacteria and inflammatory products of periodontitis can affect pulp through accessory canals, apical foramen, and dentinal tubule. Meanwhile, Vascular Endothelial Growth Factor (VEGF) and Nerve Growth Factor (NGF) are components of growth factors which increase in the inflammatory tissues. This study aimed to evaluate periodontal disease effects on the expression of VEGF and NGF in dental pulp. 
Materials and Methods: In this analytical cross-sectional study, 30 human dental pulp specimens were collected from 15 teeth with severe chronic periodontitis and 15 teeth with healthy periodontium. The percentage of stained cells (LI) and intensity of stained cells (VEGF and NGF) were calculated and compared between two groups by Independent t test and Fisher analysis.
Results: The percentage of stained cells for VEGF in the case group was 34.514 and in the control group 35.473. The percentage of stained cells for NGF in the case group was 34.962 and in the control group 34.592. There was no statistically significant difference between two groups (P>0.05). In addition, there was no significant and qualitative difference between the case and control groups with regard to the intensity of stained cells (P>0.05). 
Conclusion: According to the results of this study, severe periodontitis did not affect the expression of the VEGF and NGF in dental pulp.
Full-Text [PDF 1755 kb]   (651 Downloads) |   |   Full-Text (HTML)  (1037 Views)  
Type of Study: Original article | Subject: Pathology
Received: 2017/10/14 | Accepted: 2018/01/27 | Published: 2018/03/1

1. Seltzer S, Bender IB. Biologic Consideration of Pulp. Texas: Ishizaku Euro America; 1990. [PMCID]
2. Sadeghi R, Nazari Moghaddam K, Jooyandeh J. [Clinical evaluation of endodotic therapy on periodontal tissue healing in chronic advanced periodontitis (Persian)]. Journal of Dental Medicine. 2004; 17(3):83-91.
3. Cohen S, Hargreaves KM. Pathways of the pulp. Maryland Heights: Mosby; 2006.
4. Walton RE, Garnick JJ. The histology of periapical inflammatory lesions in permanent molars in monkeys. Journal of Endodontics. 1986; 12(2):49-53. [DOI:10.1016/S0099-2399(86)80127-3] [DOI:10.1016/S0099-2399(86)80127-3]
5. Fouad A, Torabinejad M, Walton RE. Endodontics-E-Book: Principles and practice. Amsterdam: Elsevier Health Sciences; 2008.
6. Unlu F,Guneri PG,Hekimgil M,Yesilbek B,Boyacioglu H. Expression of vascular endothelial growth factor in human periodontal tissue : Comparison of healthy and diabetic patient. Journal of Periodontology. 2003 ; 74(2):181-7. [DOI:10.1902/jop.2003.74.2.181] [PMID] [DOI:10.1902/jop.2003.74.2.181]
7. Grando ML, Westphalen BL, de Figueiredo J, Nör J, de Araujo F, Fossati A. Vascular endothelial growth factor and its relationship with the dental pulp. Journal of Endodontics. 2007; 33(5):524-30. [DOI:10.1016/j.joen.2007.01.003] [PMID] [DOI:10.1016/j.joen.2007.01.003]
8. O'Hara AH, Sampson WJ, Dreyer CW, Pierce AM, Ferguson IA. Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement. Archives of Oral Biology. 2009; 54(9):871-8. [DOI:10.1016/j.archoralbio.2009.06.003] [PMID] [DOI:10.1016/j.archoralbio.2009.06.003]
9. Chesa P, Rettig W, Thomson T, Old L, Melamed M. Immunohistochemical analysis of nerve growth factor receptor expression in normal and malignant human tissues. The Journal of Histochemistry and Cytochemistry. 1988; 36(4):383-9. [DOI:10.1177/36.4.2831267] [PMID] [DOI:10.1177/36.4.2831267]
10. Nanci A, Ten Cate AR. Ten cate's oral histology-pageburst on vitalsource: Development, structure, and function. Amsterdam: Elsevier Health Sciences; 2008. [PMCID]
11. Rubach WC, Mitchell DF. Periodontal disease, accessory canals and pulp pathosis. Journal of Periodontology. 1965; 36(1):34-8. [DOI:10.1902/jop.1965.36.1.34] [DOI:10.1902/jop.1965.36.1.34]
12. Seltzer S, Bender IB. The dental pulp: Biologic considerations in dental procedures. Philadelphia: Lippincott Williams & Wilkins; 1975.
13. Seyed Rezaei M, Khosravi M, Bijani A, Babak M. [The Effect of Pathology of Advanced Periodontal Disease on Dental Pulps (Persian)]. Journal of Dentistry. 2012; 12(3):233-8.
14. Kamali A, Disfani R, Mirzaei M. [Histopathologic study of the effects of advanced periodontal diseases on pulp (Persian)]. Journal of Islamic Dental Association of Iran. 2003; 14(3):30-6.
15. Johnson R, Serio F, Dai X. Vascular endothelial growth factors and progression of periodontal diseases. Journal of Periodontology. 1999; 70(8):848-52. [DOI:10.1902/jop.1999.70.8.848] [PMID] [DOI:10.1902/jop.1999.70.8.848]
16. Torabinejad M, Kiger R. A histologic evaluation of dental pulp tissue of a patient with periodontal disease. Oral Surgery, Oral Medicine, and Oral Pathology. 1985; 59(2):198-200. [DOI:10.1016/0030-4220(85)90018-0] [DOI:10.1016/0030-4220(85)90018-0]
17. Laurina Z, Pilmane M, Care R. Growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Stomatologija. 2009; 11(2):48-54. [PMID]
18. Harrington GW, Steiner DR, Ammons Jr WF. The periodontal–endodontic controversy. Periodontology 2000. 2002; 30(1):123-30. [PMID] [DOI:10.1034/j.1600-0757.2002.03012.x]
19. Rotstein I. Interaction between endodontics and periodontics. Periodontology 2000. 2017; 74(1):11-39. [DOI:10.1111/prd.12188] [PMID] [DOI:10.1111/prd.12188]
20. Czarnecki R, Schilder H. A histological evaluation of the human pulp in teeth with varying degrees of periodontal disease. Journal of ُndodontics. 1979; 5(8):242-53. [DOI:10.1016/S0099-2399(79)80018-7] [DOI:10.1016/S0099-2399(79)80018-7]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Journal of Dentomaxillofacial

Designed & Developed by : Yektaweb