Volume 7, Issue 1 (Spring 2018)                   3dj 2018, 7(1): 7-12 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shalchi M, Hajian-Tilaki A, khanjani M S, Sabzgolin P, Aghajani Nargesi R. Comparing Streptococcus Mutans Adhesion by Using Different Orthodontic Bracket Ligations: An In Vitro Study. 3dj. 2018; 7 (1) :7-12
URL: http://3dj.gums.ac.ir/article-1-303-en.html
1- Department of Orthodontics, Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
2- Postgraduate Student of Orthodontics, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
3- Doctor of Medical Laboratory Science, Reference Lab, Guilan University of Medical Sciences, Rasht, Iran.
4- Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
5- Department of Prosthodontics, Faculty of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
Abstract:   (143 Views)
Introduction: Orthodontic treatments and brackets application with various ligature methods have increased worldwide, but the growth rate and mechanism of microorganism adhesion to these ligatures is not fully discovered. The current study aimed to compare the level of streptococcus mutans adhesion to three different ligation methods.
Materials and Methods: This in vitro study was performed on 30 samples. Three different ligature methods were used and 10 samples were used in each group. In group A, conventional brackets with elastomeric ligature, in group B, conventional brackets with steel wire ligature and in group C, self-ligated brackets were used. Resin composite was condensed on the mesh surface of the brackets and cured for 40 seconds. Then, the coated samples with saliva were put into glass vials, immersed in 2 mL of streptococcus mutans suspension (×109 CFU) and incubated at 37ºC for 24 h. Then, the samples were washed 3 times with normal saline, immersed into 2 mL of normal saline and shaked for 2 min. The obtained suspension was cultured on blood agar incubated at 37ºC for 48 h and the formed colonies counted. In analysis, we performed 1-way analysis of variance with Tukey test with multiple comparison in SPSS version 16. 
Results: According to the results, streptococcus mutans growth rate showed statistically significant differences in the three groups. It was minimum in the steel wire (23.80±1.40), and maximum in elastomeric ligatures (38.60±1.84).
Conclusion: Steel wire ligatures had better effect on decreasing bacterial adhesion. The findings high light to decrease the use of elastomeric ligation brackets in patients with poor oral hygiene. 
Full-Text [PDF 631 kb]   (33 Downloads) |   |   Full-Text (HTML)  (29 Views)  
Type of Study: Original article | Subject: Pathology
Received: 2017/09/10 | Accepted: 2018/01/5 | Published: 2018/03/1

References
1. Lombardo L, Ortan YÖ, Gorgun Ö, Panza C, Scuzzo G, Siciliani G. Changes in the oral environment after placement of lingual and labial orthodontic appliances. Progress in Orthodontics. 2013; 14:28. [DOI:10.1186/2196-1042-14-28] [PMID] [PMCID] [DOI:10.1186/2196-1042-14-28]
2. Harikrishnan P, Subha TS, Kavitha V, Gnanamani A. Microbial adhesion on orthodontic ligating materials: An in vitro assessment. Advances in Microbiology. 2013; 3(1):108-14. [DOI:10.4236/aim.2013.31017] [DOI:10.4236/aim.2013.31017]
3. Nascimento LE, Souza MM, Azevedo AR, Maia LC. Are self-ligating brackets related to less formation of Streptococcus mutans colonies? A systematic review. Dental Press Journal of Orthodontics. 2014; 19(1):60-8 [DOI:10.1590/2176-9451.19.1.060-068.oar] [PMCID] [DOI:10.1590/2176-9451.19.1.060-068.oar]
4. Brusca M, Chara O, Sterin Borda L, Rosa A. Influence of different orthodontic brackets on adherence of microorganisms in vitro. The Angle Orthodontist. 2007; 77(2):331-6. [DOI:10.2319/0003-3219(2007)077[0331:IODOBO]2.0.CO;2]
5. Pithon MM, dos Santos RL, Nascimento LE, Ayres AO, Alvian D, Bolognese Am. Do self-ligating brackets favor greater bacterial aggregation? Brazilian Journal of Oral Sciences. 2011; 10(3):208-12. [DOI:10.20396/bjos.v10i3.8641633]
6. Pejda S, Varga ML, Milosevic SA, Mestrovic S, Slaj M, Repic D, et al. Clinical and microbiological parameters in patients with self-ligating and conventional brackets during early phase of orthodontic treatment. The Angle Orthodontist. 2012; 83(1):133-9. [DOI:10.2319/010412-8.1] [PMID] [DOI:10.2319/010412-8.1]
7. de Souza RA, de Araújo Magnani MB, Nouer DF, da Silva CO, Klein MI, Sallum EA, et al. Periodontal and microbiologic evaluation of 2 methods of archwire ligation: Ligature wires and elastomeric rings. American Journal of Orthodontics and Dentofacial Orthopedics. 2008; 134(4):506-12. [DOI:10.1016/j.ajodo.2006.09.067] [PMID] [DOI:10.1016/j.ajodo.2006.09.067]
8. Gameiro GH, Nouer DF, Cenci MS, Cury JA. Enamel demineralization with two forms of archwire ligation investigated using an in situ caries model-a pilot study. The European Journal of Orthodontics. 2009; 31(5):542-6. [DOI:10.1093/ejo/cjn119] [PMID] [DOI:10.1093/ejo/cjn119]
9. Ahmed I, Saif ul Haque, Nazir R. Carious lesions in patients undergoing orthodontic treatment. Journal of Pakistan Medical Association. 2011; 61(12):1176-9. [PMID] [PMID]
10. Garcez AS, Suzuki SS, Ribeiro MS, Mada EY, Freitas AZ, Suzuki H. Biofilm retention by 3 methods of ligation on orthodontic brackets: A microbiologic and optical coherence tomography analysis. American Journal of Orthodontics and Dentofacial Orthopedics. 2011; 140(4):193-8. [DOI:10.1016/j.ajodo.2011.04.019] [PMID] [DOI:10.1016/j.ajodo.2011.04.019]
11. Batoni G, Pardini M, Giannotti A, Ota F, Rita Giuca M, Gabriele M, et al. Effect of removable orthodontic appliances on oral colonisation by mutans streptococci in children. European Journal of Oral Sciences. 2001; 109(6):388-92. [DOI:10.1034/j.1600-0722.2001.00089.x] [PMID] [DOI:10.1034/j.1600-0722.2001.00089.x]
12. Shashidhar EP, Sahitya M, Sunil T, Murthy AR, Rani MS. A comparative evaluation of adherence of microorganism to different types of brackets: A scanning electron microscopic study. Journal of International Oral Health. 2015; 7(9):25-30. [PMID] [PMCID] [PMID] [PMCID]
13. Gastel J, Quirynen M, Teughels W, Pauwels M, Coucke W, Carels C. Microbial adhesion on different bracket types in vitro. The Angle Orthodontist. 2009; 79(5):915-21. [DOI:10.2319/092908-507.1] [PMID] [DOI:10.2319/092908-507.1]
14. Forsberg CM, Brattström V, Malmberg E, Nord CE. Ligature wires and elastomeric rings: Two methods of ligation, and their association with microbial colonization of Streptococcus mutans and iactobacilli. The European Journal of Orthodontics. 1991; 13(5):416-20. [DOI:10.1093/ejo/13.5.416] [DOI:10.1093/ejo/13.5.416]
15. Türkkahraman H, Sayın M, Bozkurt FY, Yetkin Z, Kaya S, Önal S. Archwire ligation techniques, microbial colonization, and periodontal status in orthodontically treated patients. The Angle Orthodontist. 2005; 75(2):231-6. [PMID] [PMID]
16. Raju AS, Hegde NA, Reddy VP, Chandrashekar B, Mahendra S, Harishkoushik S. An in vivo study on bacterial colonization with metal, ceramic and self-ligating brackets: A scanning electron microscopy study. Journal of Indian Orthodontic Society. 2013; 47(2):88-96 [DOI:10.5005/jp-journals-10021-1135] [DOI:10.5005/jp-journals-10021-1135]
17. do Nascimento LE, Pithon MM, dos Santos RL, Freitas AO, Alviano DS, Nojima LI, et al. Colonization of Streptococcus mutans on esthetic brackets: Self-ligating vs conventional. American Journal of Orthodontics and Dentofacial Orthopedics. 2013; 143(4):S72-7. [DOI:10.1016/j.ajodo.2012.07.017] [PMID] [DOI:10.1016/j.ajodo.2012.07.017]

Add your comments about this article : Your username or Email:
CAPTCHA code

© 2019 All Rights Reserved | Journal of Dentomaxillofacial

Designed & Developed by : Yektaweb